Methoden und Dienstleistungen

Routine Diagnostik

• ADAMTS13 and Von Willebrand factor (VWF) in ISO/IEC 17025 accredited laboratory (STS259)
• Molecular analysis ADAMTS13 gene (routinely) and complement protein genes (upon special request)

Forschungstätigkeiten

Various tools with focus on VWF and ADAMTS13, as well as contact phase proteins

Allgemeine Hämostase

• ADAMTS13 activity: FRETS-VWF73, quantitative immunoblotting, residual collagen binding Anti-ADAMTS13 antibodies: functional inhibitor assay, antibody
• ELISA, quantitative dot blot
• VWF: VWF activity, VWF Ag, VWF multimers

Protein Analytik

• ELISA techniques: Absorbance, fluorescence, luminescence
• Protein purification: Different chromatography techniques (ion exchange, gel filtration, affinity, FPLC, HPLC), immunoprecipitation, etc.
• Gel electrophoresis: SDS-Page, agarose, isoelectric focusing, affinity electrophoresis
• Protein / antibody labeling: Biotin/streptavidin, HRP, etc.

• Epitope mapping
• Surface Plasmon resonance
• Posttranslational modifications

Immunohistochemie, Immunofluoreszenz und verwandte Techniken

• Western blot, quantitative dot blot
• Light and confocal microscopy
• Flow cytometry and single cell sorting
• ELISPOT

Molekular Biologie

• PCR, RT-PCR, restriction analysis, Sanger sequencing and Next Generation Sequencing
• DNA electrophoresis, hybridization
• Site directed mutagenesis, expression and production of recombinant proteins

Phage and ribosomal display (iTTP)

• Antibody repertoire cloning, scFv or Fab Generation of small mimetic proteins/peptides
• Antibody engineering (subclass switching and clonal autoantibody evolution studies)

Weitere Techniken

• Long-term cell cultures of EBV transformed B- and T-cell lines

Kontaktieren Sie uns, wenn Sie sich für eine der oben genannten Techniken interessieren contact.