Welcome to the website of the
Hereditary TTP Registry
Hereditary Thrombotic Thrombocytopenic Purpura (hTTP) or Upshaw-Schulman Syndrome (USS) is an ultra-rare and complex blood coagulation disease
read more about hereditary TTP
At present, the Hereditary TTP Registry has enrolled 231 participants from 18 different countries
click here to know which sites are involved
«The fact that the medical research of a son of the Mississippi Delta, born in Louise, MS in 1929, is being continued by a team of researchers all over the world, still excites me!»

Cristie Upshaw Travis
Daughter of Dr. Jefferson D. Upshaw
The Hereditary TTP Registry participated in the first-in-human study evaluating a recombinant human ADAMTS13 as therapeutic option in the treatment of congenital TTP
click here to read more about the study

Methods & Services

Routine Diagnostics

ADAMTS13 and Von Willebrand factor (VWF) in ISO/IEC 17025 accredited laboratory (STS259)
Molecular analysis ADAMTS13 gene (routinely) and complement protein genes (upon special request)

Research Lab

Various tools with focus on VWF and ADAMTS13, as well as contact phase proteins

General hemostasis

ADAMTS13 activity: FRETS-VWF73, quantitative immunoblotting, residual collagen binding Anti-ADAMTS13 antibodies: functional inhibitor assay, antibody
ELISA, quantitative dot blot
VWF: VWF activity, VWF Ag, VWF multimers

Protein analytics

ELISA techniques: Absorbance, fluorescence, luminescence
Protein purification: Different chromatography techniques (ion exchange, gel filtration, affinity, FPLC, HPLC), immunoprecipitation, etc.
Gel electrophoresis: SDS-Page, agarose, isoelectric focusing, affinity electrophoresis
Protein / antibody labeling: Biotin/streptavidin, HRP, etc. 
Epitope mapping
Surface Plasmon resonance
Posttranslational modifications

Immunohistochemistry, immunofluorescence and related techniques

Western blot, quantitative dot blot
Light and confocal microscopy
Flow cytometry and single cell sorting
ELISPOT

Molecular biology

PCR, RT-PCR, restriction analysis, Sanger sequencing and Next Generation Sequencing
DNA electrophoresis, hybridization
Site directed mutagenesis, expression and production of recombinant proteins

Phage and ribosomal display (iTTP)

Antibody repertoire cloning, scFv or Fab Generation of small mimetic proteins/peptides
Antibody engineering (subclass switching and clonal autoantibody evolution studies)

Miscellaneous

Long-term cell cultures of EBV transformed B- and T-cell lines

If you are interested in one of the above, please contact us.